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KMID : 0613820070170070983
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Journal of Life Science
2007 Volume.17 No. 7 p.983 ~ p.988
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Confirmation of Non-Siderophore Antifugal Substance and Cellulase from Bacillus licheniformis K11 Containing Antagonistic Ability and Plant Growth Promoting Activity
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Woo Sang-Min
Kim Sang-Dal
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Abstract
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Bacillus licheniformis K11, a plant growth promoting rhizobacterium was reported as a producer of auxin, siderophore, as well as antifungal cellulase under some culture conditions. In vitro test, B. licheniformis K11 represented excellent antagonistic ability against Fusarium oxyspoum (KACC 40037), and showed broad spectrum against other phytopathogenic fungi. B. licheniformis K11 had cellulolytic activity toward not only carboxymethyl-cellulose (CMC) but also insoluble cellulose, such as fungal cell wall cellulose, filter paper (Whatman No. 1), and Avicel. In addition, we confirmed antifungal substance production by butanol-extract methods. The strain produced optimally the antifungal substance when it was cultivated at pH 9.0, 30℃ for 4 days on nutrient medium. The biological control mechanisms of B. licheniformis K11 were caused by antifungal substance, cellulase and siderophore against phytopathogenic fungi.
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KEYWORD
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PGPR(plant prowth promoting rhizobacterium), antifungal substance, cellulase, siderophore, Bacillus licheniformis K11
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